Wasps back Paolo Odogwu and Bristol scrum-half Harry Randall have won their first England call-ups for the Six Nations.
Bath prop Beno Obano is the third uncapped player to be named by Eddie Jones in the 28-man squad.
Courtney Lawes returns from injury, while Mako Vunipola, who last played in the Autumn Nations Cup final win over France, will join the squad for rehabilitation but be unavailable for selection.
Wasps flanker Jack Willis and Bath's Jonathan Joseph only make the 12-man shadow squad, while Kyle Sinckler, who is banned for the opening game against Scotland on February 6, is not in either group.
Owen Farrell will retain his position as captain.
The squad will meet up at St. George's Park on January 27, with coach Jones, who is isolating after assistant Matt Proudfoot tested positive for coronavirus, set to arrive a day later.
"This has been a really tough squad to pick and I know there are a lot of disappointed players who haven't been selected," said Jones.
"We're really excited by the players we have got, it's a very vibrant squad and are looking forward to getting started next week.
"We're very grateful to the Premiership Rugby, the clubs and RPA [Rugby Players Association] for allowing us to have 28 players throughout the tournament, we're very happy with the number and it's testament to the growing relationships between all parties.
"Ultimately our goal is to win the Six Nations and to do that we'll need to work hard and prepare well each day and take it as it comes."
Forwards: Luke Cowan-Dickie, Tom Curry, Ben Earl, Ellis Genge, Jamie George, Jonny Hill, Maro Itoje, Joe Launchbury, Courtney Lawes, Joe Marler, Beno Obano, Will Stuart, Sam Underhill, Billy Vunipola, Harry Williams, Mark Wilson.
Backs: Elliot Daly, Owen Farrell, George Ford, Ollie Lawrence, Max Malins, Jonny May, Paolo Odogwu, Harry Randall, Dan Robson, Henry Slade, Anthony Watson, Ben Youngs.
Shadow squad: Charlie Atkinson, Ali Crossdale, Tom Dunn, Charlie Ewels, George Furbank, Joe Heyes, Jonathan Joseph, Joe Marchant, George Martin, Alex Mitchell, Jacob Umaga, Jack Willis.